Anti-Cyclic Citrullinated Peptide (CCP) Antibody ELISA Cov Khoom Siv

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Cov khoom no yog npaj rau qhov zoo nyob rau hauv vitro nrhiav pom anti-cyclic citrullinated peptide antibody qib hauv tib neeg cov ntshav. Clinically, nws yog siv los ua ib qho cuab yeej pab kuaj mob rau rheumatoid mob caj dab (RA).

Anti-cyclic citrullinated peptide antibodies yog autoantibodies uas tsom cyclic citrullinated peptides, ib hom hloov kho protein antigen. Lawv lub xub ntiag yog cuam tshuam nrog kev mob caj dab rheumatoid, ib tus kab mob autoimmune ntev uas tshwm sim los ntawm kev sib koom ua ke thiab kev puas tsuaj. Piv nrog rau lwm cov kab mob rheumatoid, cov tshuaj tiv thaiv no qhia qhov tshwj xeeb rau RA, tshwj xeeb tshaj yog nyob rau theem pib ntawm tus kab mob thaum kho cov tsos mob tseem tsis tau raug.

Rau cov neeg mob uas xav tias RA, kuaj pom cov tshuaj tiv thaiv cyclic citrullinated peptide cov tshuaj tiv thaiv kab mob tuaj yeem pab kom paub meej tias qhov kev kuaj mob thaum ntxov, uas yog qhov tseem ceeb rau kev cuam tshuam raws sij hawm thiab tiv thaiv kev puas tsuaj tsis sib haum. Nws kuj tseem pab kom paub qhov txawv ntawm RA los ntawm lwm hom mob caj dab nrog cov tsos mob zoo sib xws, yog li coj cov kws kho mob los tsim cov phiaj xwm kho mob ntau dua thiab txhim kho kev tswj hwm tag nrho ntawm tus kab mob.

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Khoom cim npe

Txoj Cai

Cov khoom siv no kuaj pom cov tshuaj tiv thaiv-cyclic citrullinated peptide antibodies (CCP cov tshuaj tiv thaiv) hauv tib neeg cov ntshav kuaj raws li txoj hauv kev tsis ncaj ncees, nrog rau kev ua kom huv si cyclic citrullinated peptide antigens siv los ua cov txheej txheem antigen.

Cov txheej txheem kev sim pib nrog kev ntxiv cov qauv ntshav rau cov tshuaj tiv thaiv qhov dej uas tau ua ntej-coated nrog cov lus hais saum toj no purified antigens, ua raws li lub sijhawm incubation. Thaum lub sij hawm incubation no, yog tias CCP cov tshuaj tiv thaiv muaj nyob rau hauv cov qauv, lawv yuav paub tshwj xeeb thiab khi rau cyclic citrullinated peptide antigens coated rau ntawm microwells, tsim cov antigen-antibody complexes ruaj khov. Txhawm rau kom ntseeg tau qhov tseeb ntawm cov kauj ruam tom ntej, cov khoom tsis sib xws hauv cov tshuaj tiv thaiv qhov dej raug tshem tawm los ntawm cov txheej txheem ntxuav, uas yuav pab tshem tawm qhov cuam tshuam los ntawm lwm yam tshuaj hauv cov ntshav.

Tom ntej no, enzyme conjugates ntxiv rau cov tshuaj tiv thaiv qhov dej. Tom qab qhov thib ob incubation, cov enzyme conjugates yuav tshwj xeeb txuas rau cov antigen-antibody complexes uas twb muaj lawm, tsim kom muaj lub cev tiv thaiv kab mob loj dua uas suav nrog antigen, antibody, thiab enzyme conjugate. Thaum TMB substrate tov yog nkag mus rau hauv lub system, lub enzyme nyob rau hauv lub conjugate catalyzes cov tshuaj tiv thaiv nrog lub TMB substrate, uas ua rau ib tug pom kev hloov xim. Qhov kev siv ntawm cov tshuaj tiv thaiv xim no yog ncaj qha ntsig txog tus nqi ntawm CCP cov tshuaj tiv thaiv tam sim no hauv cov qauv ntshav hauv thawj. Thaum kawg, tus nyeem ntawv microplate yog siv los ntsuas qhov absorbance (A tus nqi) ntawm cov tshuaj tiv thaiv sib tov. Los ntawm kev txheeb xyuas tus nqi absorbance no, theem ntawm CCP cov tshuaj tiv thaiv kab mob hauv cov qauv tuaj yeem txiav txim siab kom raug, muab lub hauv paus txhim khu kev qha rau kev kuaj mob thiab kuaj mob.

Khoom nta

High rhiab heev, tshwj xeeb thiab stability

Khoom Specification

Txoj Cai	Enzyme txuas immunosorbent assay
Hom	Tsis ncajTxoj kev
Daim ntawv pov thawj	NMPA
Cov qauv	Tib neeg cov ntshav / ntshav
Specification	48T / hli96T
Cia kub	2-8℃
Txee lub neej	12hli