Anti-Endometrial (EM) Antibody ELISA Cov Khoom Siv

Cov khoom siv no kuaj pom cov tshuaj tiv thaiv endometrial antibodies (IgG) hauv tib neeg cov ntshav kuaj raws li txoj kev tsis ncaj ncees, nrog rau cov tshuaj purified endometrial membrane antigens siv rau pre-txheej microwells.

Cov txheej txheem kuaj pib los ntawm kev ntxiv cov qauv ntshav rau cov tshuaj tiv thaiv antigen-precoated qhov dej rau incubation. Yog hais tias cov tshuaj tiv thaiv endometrial muaj nyob rau hauv cov qauv, lawv yuav tshwj xeeb khi rau pre-coated endometrial antigens nyob rau hauv lub microwells, tsim ruaj khov antigen-antibody complexes. Tom qab tshem tawm cov khoom tsis sib haum los ntawm kev ntxuav kom tsis txhob cuam tshuam, horseradish peroxidase-labeled nas tiv thaiv tib neeg IgG tshuaj tiv thaiv ntxiv.

Tom qab lwm qhov incubation, cov enzyme-labeled antibodies khi rau cov antigen-antibody complexes uas twb muaj lawm. Thaum TMB substrate ntxiv, cov tshuaj tiv thaiv xim tshwm sim nyob rau hauv catalysis ntawm enzyme. Thaum kawg, tus nyeem ntawv microplate ntsuas qhov nqus (A tus nqi), uas yog siv los txiav txim seb muaj cov tshuaj tiv thaiv endometrial (IgG) hauv cov qauv.