Anti-Zona Pelllucida (ZP) Antibody ELISA Cov Khoom Siv

Lus piav qhia luv luv:

Cov khoom no yog tsim los rau qhov zoo nyob rau hauv vitro nrhiav kom tau zona pellucida (ZP) antibody qib hauv tib neeg cov ntshav. Lub zona pellucida, tshwj xeeb extracellular matrix nyob ib ncig ntawm lub oocyte, plays lub luag hauj lwm tseem ceeb nyob rau hauv cov phev paub, khi, thiab fertilization, nrog rau thaum ntxov embryonic kev loj hlob.

ZP cov tshuaj tiv thaiv yog autoantibodies uas tsom zona pellucida antigens. Thaum lawv tshwm sim hauv lub cev, lawv tuaj yeem khi tshwj xeeb rau zona pellucida, thaiv kev sib cuam tshuam ntawm cov phev thiab oocytes, yog li cuam tshuam rau kev yug menyuam. Tsis tas li ntawd, lawv tuaj yeem cuam tshuam nrog cov txheej txheem implantation ntawm fertilized qe, uas yog ib qho tseem ceeb ua rau autoimmune infertility.

Clinically, qhov kev tshawb pom no muaj feem xyuam rau txoj kev kuaj mob rau autoimmune infertility. Los ntawm kev txheeb xyuas cov qib ZP cov tshuaj tiv thaiv hauv cov neeg mob cov ntshav, nws tuaj yeem muab cov ntaub ntawv tseem ceeb rau kev qhia meej txog qhov ua rau muaj menyuam tsis taus hauv qee cov neeg mob uas tsis paub qhov ua rau, pab kws kho mob tsim cov phiaj xwm kuaj mob thiab kho mob ntau dua.

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Khoom cim npe

Txoj Cai

Cov khoom siv no kuaj pom zona pellucida cov tshuaj tiv thaiv kab mob (ZP-Ab) hauv tib neeg cov ntshav kuaj raws li txoj kev tsis ncaj ncees, nrog rau cov tshuaj purified zona pellucida siv los ua cov txheej txheem antigen.

Cov txheej txheem kev sim pib los ntawm kev ntxiv cov qauv ntshav rau cov tshuaj tiv thaiv qhov dej ua ntej coated nrog antigen, tom qab ntawd incubation. Yog tias ZP-Ab tam sim no nyob rau hauv cov qauv, nws yuav tshwj xeeb khi rau cov coated zona pellucida antigen hauv cov qhov dej, tsim cov antigen-antibody complexes ruaj khov.

Tom qab ntawd, cov enzyme conjugates ntxiv rau hauv qhov dej. Tom qab qhov thib ob incubation kauj ruam, cov enzyme conjugates khi rau cov antigen-antibody complexes uas twb muaj lawm. Thaum TMB substrate tov yog qhia, cov tshuaj tiv thaiv xim tshwm sim nyob rau hauv lub catalytic kev txiav txim ntawm lub enzyme nyob rau hauv lub complex. Thaum kawg, tus nyeem ntawv microplate yog siv los ntsuas qhov nqus (A tus nqi), uas tso cai rau kev txiav txim siab ntawm ZP-Ab qib hauv cov qauv.

Khoom nta

High rhiab heev, tshwj xeeb thiab stability

Khoom Specification

Txoj Cai	Enzyme txuas immunosorbent assay
Hom	Tsis ncajTxoj kev
Daim ntawv pov thawj	NMPA
Cov qauv	Tib neeg cov ntshav / ntshav
Specification	48T / hli96T
Cia kub	2-8℃
Txee lub neej	12hli