Enterovirus 71 (EV71) IgM ELISA Kit

Enterovirus 71 (EV71) IgM ELISA Cov Khoom Siv Duab Duab

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Enterovirus 71 IgM (EV71-IgM) ELISA cov khoom siv yog ib qho enzyme-linked immunosorbent assay rau qhov zoo ntawm IgM-class antibodies rau Enterovirus 71 hauv tib neeg cov ntshav los yog ntshav.Nws yog npaj los siv rau hauv cov chaw kuaj mob rau kev kuaj mob thiab kev tswj xyuas cov neeg mob ntsig txog kev kis tus kabmob Enterovirus 71.

Tib neeg enterovirus 71 (EV71), tus tswv cuab tshiab tshaj plaws ntawm Enteroviridae, yog ib qho ua rau muaj kab mob ntawm tes, ko taw thiab qhov ncauj (HFMD) thiab qee zaum cuam tshuam nrog cov kab mob hauv nruab nrab ntawm lub paj hlwb.Cov kab mob feem ntau tshwm sim thaum Lub Rau Hli thiab Lub Xya Hli.Kev kis tus kab mob EV71 tuaj yeem ua rau ob qho tib si asymptomatic lossis tuaj yeem ua rau raws plab thiab pob khaus.Cov menyuam yaus hnub nyoog qis dua tsib xyoos muaj kev pheej hmoo siab kis tus kab mob hnyav dua li cov neeg laus thiab menyuam yaus.

EV71 kis los ntawm ib tus neeg mus rau lwm tus los ntawm kev sib cuag ncaj qha thiab los ntawm kev kis ntawm tes lossis cov khoom uas muaj kab mob.Qhov ntswg thiab caj pas, qaub ncaug lossis kua dej los ntawm cov hlwv tuaj yeem kis tus kab mob.

EV71 tuaj yeem raug cais tawm thiab kuaj pom hauv caj pas thiab cov quav uas siv PCR kuaj.Viral RNA tau kuaj pom hauv daim tawv nqaij vesicle kua, ntshav thiab zis.Kev kuaj mob ua ntej thiab yooj yim dua tuaj yeem ua tiav siv EV71-kev soj ntsuam serological tshwj xeeb, suav nrog kev sim tshwj xeeb rau IgM tshuaj tiv thaiv.

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Khoom cim npe

Txoj Cai

Cov khoom siv no kuaj pom Enterovirus 71 IgM antibody (EV71-IgM) hauv tib neeg cov ntshav ntshav lossis ntshav kuaj, polystyrene microwell strips yog pre-coated nrog cov tshuaj tiv thaiv rau tib neeg immunoglobulin M proteins (anti-μ saw). Kev tshuaj xyuas, IgM cov tshuaj tiv thaiv hauv cov qauv tuaj yeem raug ntes, thiab lwm yam khoom tsis sib xws (xws li cov tshuaj tiv thaiv IgG tshwj xeeb) yuav raug tshem tawm los ntawm kev ntxuav.Hauv cov kauj ruam thib ob, HRP (horseradish peroxidase)-conjugated antigens tshwj xeeb tsuas yog cuam tshuam nrog EV71 IgM cov tshuaj tiv thaiv.Tom qab ntxuav kom tshem tawm cov tsis muaj HRP-conjugate, cov tshuaj chromogen ntxiv rau hauv qhov dej.Nyob rau hauv lub xub ntiag ntawm (anti-μ) - (EV71-IgM) - (EV71-Ag-HRP) immunocomplex, tom qab ntxuav lub phaj, TMB substrate tau ntxiv rau kev tsim xim, thiab HRP txuas nrog cov complex catalyzes cov xim tsim tawm cov tshuaj tiv thaiv. txhawm rau tsim cov tshuaj xiav, ntxiv 50μL ntawm Stop Solution, thiab tig daj.Lub xub ntiag ntawm absorbance ntawm EV71-IgM antibody nyob rau hauv cov qauv yog txiav txim los ntawm ib tug microplate nyeem ntawv.

Khoom nta

High rhiab heev, tshwj xeeb thiab stability

Khoom Specification

Txoj Cai	Enzyme txuas immunosorbent assay
Hom	Txoj kev ntes
Daim ntawv pov thawj	CE
Cov qauv	Tib neeg cov ntshav / ntshav
Specification	48 t / 96 t
Cia kub	2-8 ℃
Txee lub neej	12 hli