Hepatitis D virus IgM ELISA Kit

Cov khoom siv no kuaj pom tus kab mob siab D virus IgM antibody (HDV-IgM) hauv tib neeg cov ntshav ntshav lossis ntshav kuaj, polystyrene microwell strips yog pre-coated nrog cov tshuaj tiv thaiv rau tib neeg immunoglobulin M proteins (anti-μ saw).Tom qab thawj zaug ntxiv cov ntshav los yog cov ntshav plasma kuaj, cov tshuaj tiv thaiv IgM hauv cov qauv tuaj yeem raug ntes, thiab lwm yam tsis muaj kev cuam tshuam (xws li cov tshuaj tiv thaiv IgG tshwj xeeb) yuav raug tshem tawm los ntawm kev ntxuav.Hauv cov kauj ruam thib ob, HRP (horseradish peroxidase)-conjugated antigens tshwj xeeb tsuas yog cuam tshuam nrog HDV IgM cov tshuaj tiv thaiv.Tom qab ntxuav kom tshem tawm cov tsis muaj HRP-conjugate, cov tshuaj chromogen ntxiv rau hauv qhov dej.Nyob rau hauv lub xub ntiag ntawm (anti-μ) - (HDV-IgM) - (HDV Ag-HRP) immunocomplex, tom qab ntxuav lub phaj, TMB substrate tau ntxiv rau kev tsim xim, thiab HRP txuas nrog cov complex catalyzes cov xim tsim tawm cov tshuaj tiv thaiv rau tsim cov tshuaj xiav, ntxiv 50μl ntawm Stop Solution, thiab tig daj.Lub xub ntiag ntawm qhov nqus ntawm HDV-IgM antibody hauv cov qauv tau txiav txim los ntawm tus nyeem ntawv microplate.