Herpes Simplex Virus I IgM ELISA Kit

Cov khoom siv no kuaj pom Herpes Simplex Virus I lgM antibody (HSV1-lgM) hauv tib neeg cov ntshav ntshav lossis ntshav kuaj, polystyrene microwell strips yog pre-coated nrog cov tshuaj tiv thaiv qhia rau tib neeg immunoglobulin M proteins (anti-µ saw).Tom qab thawj zaug ntxiv cov tshuaj plasma los yog ntshav kuaj, cov tshuaj tiv thaiv lgM hauv cov hnoos qeev tuaj yeem raug ntes, thiab lwm yam khoom siv tsis raug cai (xws li cov tshuaj tiv thaiv lgG tshwj xeeb) yuav raug tshem tawm los ntawm kev ntxuav.Hauv cov kauj ruam thib ob, HRP (horseradish peroxidase)-conjugated antigens tshwj xeeb tsuas yog cuam tshuam nrog HSV1 lgM cov tshuaj tiv thaiv.Tom qab ntxuav kom tshem tawm cov tsis muaj HRP-conjugate, cov tshuaj chromogen ntxiv rau hauv qhov dej.Nyob rau hauv lub xub ntiag ntawm (anti- µ) - (HSV1-lgM) - (HSV1 Ag-HRP) immunocomplex, tom qab ntxuav lub phaj, TMB substrate tau ntxiv rau kev tsim xim, thiab HRP txuas nrog cov complex catalyzes cov xim tsim tawm cov tshuaj tiv thaiv rau tsim cov tshuaj xiav, ntxiv 50 µ I ntawm Stop Solution, thiab tig daj.Lub xub ntiag ntawm qhov nqus ntawm HSV1-lgM antibody hauv cov qauv tau txiav txim los ntawm tus nyeem ntawv microplate.