Toxoplasma IgG ELISA Kit

Cov khoom siv no kuaj pom Toxoplasma lgG antibody (TOX-lgG) hauv tib neeg cov ntshav ntshav lossis ntshav kuaj, polystyrene microwell strips yog pre-coated nrog Toxoplasma antigen.Tom qab thawj zaug ntxiv cov ntshav los yog cov ntshav plasma kuaj, cov tshuaj tiv thaiv kab mob sib xws (TOX-lgG-Ab & qee qhov lgM-Ab) muaj nyob rau hauv cov neeg mob cov ntaub ntawv khi rau cov antigens ntawm cov theem khoom, thiab lwm yam khoom tsis sib haum yuav raug tshem tawm los ntawm kev ntxuav.Hauv theem ob, HRP (horseradish peroxidase)-conjugated anti-tib neeg lgG yuav tshwj xeeb tsuas yog nrog TOX lgG cov tshuaj tiv thaiv.Tom qab ntxuav kom tshem tawm cov tsis muaj HRP-conjugate, cov tshuaj chromogen ntxiv rau hauv qhov dej.Nyob rau hauv lub xub ntiag ntawm (TOX Ag) - (TOX-lgG) - (anti-tib neeg lgG-HRP) immunocomplex, tom qab ntxuav lub phaj, TMB substrate tau ntxiv rau kev tsim xim, thiab HRP txuas nrog cov complex catalyzes cov xim tsim tawm cov tshuaj tiv thaiv. los tsim cov tshuaj xiav, ntxiv 50 µ I ntawm Stop Solution, thiab tig daj.Lub xub ntiag ntawm absorbance ntawm TOX-lgG antibody nyob rau hauv cov qauv yog txiav txim los ntawm ib tug microplate nyeem ntawv.