Measles Virus (MV) IgG ELISA Kit

Cov khoom siv no kuaj pom tus kab mob Measles IgG antibody (MV-IgG) hauv tib neeg cov ntshav ntshav lossis ntshav kuaj, polystyrene microwell strips yog pre-coated nrog Measles virus antigen.Tom qab thawj zaug ntxiv cov ntshav los yog cov ntshav plasma kuaj, cov tshuaj tiv thaiv kab mob sib xws (MV-IgG-Ab & qee qhov IgM-Ab) muaj nyob rau hauv cov neeg mob cov ntaub ntawv khi rau cov antigens ntawm lub sijhawm khoom, thiab lwm yam khoom tsis sib haum yuav raug tshem tawm los ntawm kev ntxuav.Hauv theem thib ob, HRP (horseradish peroxidase)-conjugated anti-tib neeg IgG yuav tshwj xeeb tsuas yog nrog MV IgG tshuaj tiv thaiv.Tom qab ntxuav kom tshem tawm cov tsis muaj HRP-conjugate, cov tshuaj chromogen ntxiv rau hauv qhov dej.Nyob rau hauv lub xub ntiag ntawm (MV Ag) - (MV-IgG) - (anti-tib neeg IgG-HRP) immunocomplex, tom qab ntxuav lub phaj, TMB substrate tau ntxiv rau kev tsim xim, thiab HRP txuas nrog cov complex catalyzes cov xim tsim tawm cov tshuaj tiv thaiv. los tsim cov tshuaj xiav, ntxiv 50μl ntawm Nres Tshuaj, thiab tig daj.Qhov muaj qhov nqus ntawm MV-IgG antibody hauv cov qauv tau txiav txim los ntawm tus nyeem ntawv microplate.